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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81

Fig. 3

Validation of CD81-dependent transport of HCV E2 in HCV core expressing cells. a Schematic representation of the expression plasmids containing lentiviral vectors transgene sequences inducing the cellular expression of HCV Core, E1E2 and p7NS2 ORFs; b cellular expression of MLV p30, CD81, HCV E1 and HCV E2 determined by western blotting in HEK293, HEK293-HCVCore-NS2, HEK293-HCVCore-NS2 shCD81, HuH-7, HuH7-HCVCore-NS2 and HuH7-HCVCore-NS2 shCD81. c Surface displayed CD81 and HCV E2 proteins in (c) HEK293, HEK293-HCVCore-NS2, HEK293-HCVCore-NS2 shCD81 and d HuH-7, HuH7-HCVCore-NS2 and HuH7-HCVCore-NS2 shCD81

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