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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81

Fig. 2

Cellular expression of and incorporation of HCV envelope proteins in chimeric HCVpp. a Cellular expression of MLV p30, CD81, HCV E1 and HCV E2 determined by western blotting in 293rVLP, 293rVLP-HCVpp, 293rVLP-HCVpp (AEE), 293rVLP-shCD81 cells and 293rVLP-HCVpp-shCD81 (BEE); b incorporation of CD81, HCV E1 and HCV E2 membrane proteins in null retrovirus particles or HCVpp produced in 293rVLP, 293rVLP-HCVpp, 293rVLP-HCVpp (AEE), 293rVLP-shCD81 cells and 293rVLP-HCVpp (BEE) cells determined by western blotting of purified particles; c surface displayed CD81 and HCV E2 proteins 293rVLP, 293rVLP-HCVpp, 293rVLP-HCVpp (AEE), 293rVLP-shCD81 cells and 293rVLP-HCVpp (BEE) cells determined by flow cytometry

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