Synergistic effect of acetyl xylan esterase from Talaromyces leycettanus JCM12802 and xylanase from Neocallimastix patriciarum achieved by introducing carbohydrate-binding module-1

AMB Express

Table 3 Order of addition about acetyl xylan esterase and xylanase on starch-free wheat bran

Order	AXE and NPXYN11		AXE + CBM1 and NPXYN11
Order	Reducing sugar content (μmol mL⁻¹)	Percentage of promotion with order 2 (%)	Reducing sugar content (μmol/mL)	Percentage of promotion with order 2 (%)
1^a	ND		ND
2^b	5.97 ± 0.18		5.97 ± 0.18
3^c	6.91 ± 0.11	16	7.72 ± 0.21	29
4^d	6.21 ± 0.17	4	6.33 ± 0.19	6
5^e	6.51 ± 0.11	9	6.69 ± 0.02	12

^aOnly added 50 U acetyl xylan esterase at 50 °C water bath for 100 rpm at 1 h
^bOnly added 200 U xylanase at 50 °C water bath for 100 rpm at 1 h
^cAdded 50 U acetyl xylan esterase first at 50 °C water bath for 100 rpm at 1 h. Then boiled 5 min for deactivation and added 200 U xylanase next for reaction at 50 °C for 100 rpm at 1 h
^dAdded 200 U xylanase first at 50 °C water bath for 100 rpm at 1 h. Then boiled 5 min for deactivation and added 50 U acetyl xylan esterase next for reaction at 50 °C for 100 rpm at 1 h
^eSimultaneously added 50 U acetyl xylan esterase and 200 U xylanase for reaction at 50 °C water bath at 1 h for 100 rpm