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Table 3 Order of addition about acetyl xylan esterase and xylanase on starch-free wheat bran

From: Synergistic effect of acetyl xylan esterase from Talaromyces leycettanus JCM12802 and xylanase from Neocallimastix patriciarum achieved by introducing carbohydrate-binding module-1

Order AXE and NPXYN11 AXE + CBM1 and NPXYN11
Reducing sugar content (μmol mL−1) Percentage of promotion with order 2 (%) Reducing sugar content (μmol/mL) Percentage of promotion with order 2 (%)
1a ND   ND  
2b 5.97 ± 0.18   5.97 ± 0.18  
3c 6.91 ± 0.11 16 7.72 ± 0.21 29
4d 6.21 ± 0.17 4 6.33 ± 0.19 6
5e 6.51 ± 0.11 9 6.69 ± 0.02 12
  1. aOnly added 50 U acetyl xylan esterase at 50 °C water bath for 100 rpm at 1 h
  2. bOnly added 200 U xylanase at 50 °C water bath for 100 rpm at 1 h
  3. cAdded 50 U acetyl xylan esterase first at 50 °C water bath for 100 rpm at 1 h. Then boiled 5 min for deactivation and added 200 U xylanase next for reaction at 50 °C for 100 rpm at 1 h
  4. dAdded 200 U xylanase first at 50 °C water bath for 100 rpm at 1 h. Then boiled 5 min for deactivation and added 50 U acetyl xylan esterase next for reaction at 50 °C for 100 rpm at 1 h
  5. eSimultaneously added 50 U acetyl xylan esterase and 200 U xylanase for reaction at 50 °C water bath at 1 h for 100 rpm