Table 2 Oligonucleotide primers used in this study
From: Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates
Gene | Primer | Sequence | Restriction enzymes |
|---|---|---|---|
Bbr_0010 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatgcatcaccatcaccatcaccatcacc | EcoRV |
Rv | tgcgcaTCTAGAtcagatgagttcgagtgtcac | XBaI | |
Bbr_0285 | Fw | tgcatcGATATCatgcatcaccatcaccatcaccatcaccatcacatggagcgaatccaatacccc | EcoRV |
Rv | tgcgcaTCTAGAtcacacctgcacgtagccg | XBaI | |
Bbr_0310 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatggggacgacaggacacagc | EcoRV |
Rv | tgcgcaTCTAGAtcaactcttttcgattgcg | XBaI | |
Bbr_0420 | Fw | tgcatcCCCGGGcatcaccatcaccatcaccatcaccatcacatgactactcgtagagc | XmaI |
Rv | ctcgaaTCTAGActagcaggacgttttagcg | XBaI | |
Bbr_0529 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatggaacatcgcgaattcaag | EcoRV |
Rv | tgcgcaTCTAGAttacagctttaccaccagcac | XBaI | |
Bbr_1552 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatgaacacaaccgacgatcag | EcoRV |
Rv | tgcgcaTCTAGAtcagatgagttcgaggttcac | XBaI | |
Bbr_1689-1690 | Fw | tgcatcCAGCTGcatcaccatcaccatcaccatcaccatcacatgagcaagcagaacgattg | PvuII |
Rv | tgcgcaTCTAGAgctggcatcttcctgaacg | XBaI | |
B7017_2031 | Fw | tgcatcGAATTCcatcaccatcaccatcaccatcaccatcacatgaccgacaccatggcacacacacaacc | EcoRI |
Rv | tgcatcTCTAGActgatgatgaaggatgactgaagccg | XBaI | |
B216_06500 | Fw | tgcatc ATTTAAAT atg catcaccatcaccatcaccatcaccatcac gtcaataccgttagggttgt | SwaI |
Rv | tgcatc TCTAGAcccggggagactcgcgagagt | XBaI | |
B216_08266 | Fw | tgcatcATTTAAATcatcaccatcaccatcaccatcaccatcacatgagtaaacgcagaaagcacag | SwaI |
Rv | tgcgcaTCTAGAgtatgtcgcgtgtcaccg | XBaI | |
B216_08730 | Fw | tgcatcGATATCatgcatcaccatcaccatcaccatcaccatcacgtgcgcgcgcgacgtgactttg | EcoRV |
Rv | tgcatc TCTAGA aacgttgaaatagagccggaaac | XBaI | |
B216_09411 | Fw | tgcatc ATTTAAATatg catcaccatcaccatcaccatcaccatcac ttcattccccggtactacg | SwaI |
Rv | tgcatc TCTAGA atccgatacccgtacccgtg | XBaI | |
B216_09623 | Fw | tgcatcATTTAAATcatcaccatcaccatcaccatcaccatcacatgaacacaaccgacgatcagc | SwaI |
Rv | tgcgcaTCTAGAatgagcgagaggacctggcg | XBaI | |
B8809_0321 | Fw | tgcatcATTTAAATcatcaccatcaccatcaccatcaccatcacatgactactcatagagcatttag | SwaI |
Rv | tgcatc TCTAGAcattctagcgcggtttag | XBaI | |
B8809_0415 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatggaacgtaaagagttcaagtgg | EcoRV |
Rv | tgcatcTCTAGAccgttgggtaattaggcgct | XBaI | |
B8809_0611 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatgacagacgtcacacatgtcg | EcoRV |
Rv | tgcatc TCTAGAtgcacggtggactatcggatc | XBaI | |
B8809_1361 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatgcagcatcccatccccaccac | EcoRV |
Rv | tgcatc TCTAGAcagcacgataaagaagctccctcg | XBaI | |
Blon_2016 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatggaacatagagcgttcaagt | EcoRV |
Rv | tgcatcTCTAGAcggctccctgctgcgatga | XBaI | |
Blon_2123 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatggtgcgtgcgcgacgtgactt | EcoRV |
Rv | tgcatcTCTAGAaccatgtacgtcggcaccgt | XBaI | |
Blon_2416 | Fw | tgcatcGATATCcatcaccatcaccatcaccatcaccatcacatgaccgacaccatggcaca | EcoRV |
Rv | tgcatcTCTAGAcggttgctgacttgggatat | XBaI |