Table 2 Experimental Box–Behnken design (BBD) with the actual values of the independent factors inoculum size (A), temperature (B) and pH (C) and the observed and predicted responses
Run no. | Inoculum size (%) | Temperature (°C) | pH | Response RL concentration (g/l) | |
|---|---|---|---|---|---|
Observed | Predicted | ||||
1 | 1 | 30 | 7 | 39.15 ± 0.84 | 41.78 |
2 | 2 | 30 | 8 | 43.85 ± 0.28 | 44.54 |
3 | 2 | 30 | 6 | 35.75 ± 1.63 | 35.53 |
4 | 5 | 30 | 7 | 34.15 ± 0.83 | 34.80 |
5 | 1 | 33.5 | 8 | 38.70 ± 1.35 | 38.23 |
6 | 1 | 33.5 | 6 | 32.40 ± 2.05 | 29.22 |
7 | 2 | 33.5 | 7 | 36.35 ± 0.98 | 31.98 |
8 | 5 | 33.5 | 8 | 29.95 ± 0.03 | 31.25 |
9 | 5 | 33.5 | 6 | 23.00 ± 0.30 | 22.24 |
10 | 1 | 37 | 7 | 23.50 ± 1.00 | 25.68 |
11 | 2 | 37 | 8 | 30.10 ± 0.55 | 28.44 |
12 | 2 | 37 | 6 | 15.40 ± 0.20 | 19.43 |
13 | 5 | 37 | 7 | 19.50 ± 0.50 | 18.70 |