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Table 2 Experimental Box–Behnken design (BBD) with the actual values of the independent factors inoculum size (A), temperature (B) and pH (C) and the observed and predicted responses

From: Rhamnolipid production by a gamma ray-induced Pseudomonas aeruginosa mutant under solid state fermentation

Run no. Inoculum size (%) Temperature (°C) pH Response RL concentration (g/l)
Observed Predicted
1 1 30 7 39.15 ± 0.84 41.78
2 2 30 8 43.85 ± 0.28 44.54
3 2 30 6 35.75 ± 1.63 35.53
4 5 30 7 34.15 ± 0.83 34.80
5 1 33.5 8 38.70 ± 1.35 38.23
6 1 33.5 6 32.40 ± 2.05 29.22
7 2 33.5 7 36.35 ± 0.98 31.98
8 5 33.5 8 29.95 ± 0.03 31.25
9 5 33.5 6 23.00 ± 0.30 22.24
10 1 37 7 23.50 ± 1.00 25.68
11 2 37 8 30.10 ± 0.55 28.44
12 2 37 6 15.40 ± 0.20 19.43
13 5 37 7 19.50 ± 0.50 18.70