AMB Express

Table 1 Levels for variables in the central composite design experiments

From: Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

Variable	Level
Variable	− α	− 1	0	+ 1	+ α
Temperature (°C)	30.6	25.0	30.3	35.5	39.9
OD₆₀₀ at induction	0.29	0.50	0.75	1.00	1.21
[Arabinose] (% w/v)	0.000	0.059	0.130	0.200	0.259

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