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AMB Express

Table 1 Levels for variables in the central composite design experiments

From: Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

Variable Level
− α − 1 0 + 1 + α
Temperature (°C) 30.6 25.0 30.3 35.5 39.9
OD600 at induction 0.29 0.50 0.75 1.00 1.21
[Arabinose] (% w/v) 0.000 0.059 0.130 0.200 0.259
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