Fig. 5
From: Partial purification and characterization of phytase from Aspergillus foetidus MTCC 11682
Coomassie Blue stained 10% SDS-PAGE gel image showing the protein profiles under A reducing B non-reducing conditions c zymogram for activity bands of partially purified protein eluted by Sephacryl S-200HR column. In panel (A), Lane 1 is the standard molecular weight (MW) marker protein and lane 2 and 3 represents the peak II and III proteins subjected to 2 Mercaptoethanol treatment. Shifting of the high intense band in lane 3 indicated the presence of disulfide bond in the protein. In B, lane 1 and 2 represents peak II and III proteins without 2 ME treatment. C represents the zymogram showing the phytase activity. Lane 1 clearance zone denoted by positive standard phytase (E. coli produced recombinant phytase of A niger) and lane 2, though the clearance zone could not be captured, the phytase activity band was observed at the arrow head