Fig. 1

Elution pattern of about 20 mg ammonium sulphate precipitated proteins from fungal spent culture in Sephacryl S-200HR column (95 cm × 1.6 cm) and flow rate of 1 mL per min. The column was equilibrated and eluted in 0.1 M sodium acetate buffer, pH 5.0. All the tubes were monitored for 280 nm absorbance for protein and enzyme activity. Standard protein (BSA) dimer (~ 136 kDa) and monomer (~ 68 kDa) was used to understand the approximate MW range where the phytase active protein eluted