Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos

AMB Express

Table 2 Numbers of sequences analyzed for the two samples

Regions	Samples	Reads	OTU	Ace	Chao	Shannon	Simpson	Coverage	Observed species
V1V2V3	A1	17,349	133	133.51	134.99	1.83	0.637187	0.9981	127
V1V2V3	B1	9763	88	89.39	89.52	2.58	0.448405	0.9994	88
V3V4	A1	18,879	175	178.99	179.67	3.21	0.362518	0.9995	173
V3V4	B1	21,213	170	171.17	170.37	3.83	0.203652	0.9992	168
V4V5	A1	35,591	211	216.33	214.51	2.64	0.466091	0.9995	209
V4V5	B1	39,179	259	259.77	260.03	3.46	0.319225	0.9997	258
V5V6V7	A1	23,913	230	235.58	234.11	5.59	0.038895	0.9989	226
V5V6V7	B1	21,074	233	236.45	238.65	5.41	0.046749	0.9993	232

Operational Taxonomic Units (OTUs), estimated OTU richness, sample coverage, and diversity indices of Shannon and Simpson were calculated from 16S rRNA gene sequences of two samples