Skip to main content

Table 1 Primers used in current study

From: Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos

Primer pairs

Primer sequence (5′–3′)

M

References

8F

TGGAGAGTTTGATCCTGGCTCAG

V1V2V3

Sun et al. (2014)

533R

TACCGCGGCTGCTGGCAC

336F

GTACTCCTACGGGAGGCAGCA

V3V4

Munyaka et al. (2015)

806R

GTGGACTACHVGGGTWTCTAAT

515F

GTGCCAGCMGCCGCGGTAA

V4V5

Tuan et al. (2014)

909R

CCCCGYCAATTCMTTTRAGT

799F

AACMGGATTAGATACCCKG

V5V6V7

Beckers et al. (2016)

1193R

ACGTCATCCCCACCTTCC

  1. Primers are indicated as forward (F) or reverse (R)
  2. M, Hypervariable region of the 16S rRNA operon targeted by primer pairs