Table 2 Primers used in this study
From: Correction to: Autolysis of Pichia pastoris induced by cold
Primera | Sequence (5′→3′)b | Restriction enzyme | Underlined sequencec |
|---|---|---|---|
YL1-F | GGGGtcgcgaAATCTTCCTCAGAAGAGGAAT | NruI | Homologous Pcctα region corresponding to nucleotides (nt) 886843 to 886863 |
YL1-R | GCGCggatccTGAATAATTGGGACATCGCTAA | BamHI | Homologous Pcctα region corresponding to nt 887226 to 887247 |
YL2-F | CCCCagatctTTCTCTCGTTAGCTTCCCAAA | BglII | Homologous eng region corresponding to nt 802080 to 802100 |
YL2-R | GCGCctcgagGTCAACTATGTGTGGTACCTT | XhoI | Homologous eng region corresponding to nt 805196 to 805216 |
YL3-F | GCGCgtcgacTGAGTTTTAGCCTTAGACATGACT | SalI | Homologous aox1 TT region corresponding to nt 890 to 913 |
YL3-R | GGGGatttaaatGGGGATCCGCACAAACGAA | SwaI | Homologous aox1 TT region corresponding to nt 1217 to 1235 |
YL4-F | CCCCatttaaatGGATCCCCCACACACCATA | SwaI | Homologous Ptef1 region corresponding to nt 965 to 983 |
YL4-R | GCGCcacgtgCACATGTTGGTCTCCAGCTT | PmlI | Homologous cyc1 region corresponding to nt 2157 to 2138 |
YL5-F | GGGGgccggcCCAAGTCCAAGGTCTCCAAT | NaeI | Homologous leu2 region corresponding to nt 86424 to 86443 |
YL5-R | GGGGcacgtgTTTCCGAACCTTACAGTAGAG | PmlI | Homologous leu2 region corresponding to nt 87940 to 87920 |