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Table 2 Primers used in this study

From: Correction to: Autolysis of Pichia pastoris induced by cold

Primera Sequence (5′→3′)b Restriction enzyme Underlined sequencec
YL1-F GGGGtcgcgaAATCTTCCTCAGAAGAGGAAT NruI Homologous Pcctα region corresponding to nucleotides (nt) 886843 to 886863
YL1-R GCGCggatccTGAATAATTGGGACATCGCTAA BamHI Homologous Pcctα region corresponding to nt 887226 to 887247
YL2-F CCCCagatctTTCTCTCGTTAGCTTCCCAAA BglII Homologous eng region corresponding to nt 802080 to 802100
YL2-R GCGCctcgagGTCAACTATGTGTGGTACCTT XhoI Homologous eng region corresponding to nt 805196 to 805216
YL3-F GCGCgtcgacTGAGTTTTAGCCTTAGACATGACT SalI Homologous aox1 TT region corresponding to nt 890 to 913
YL3-R GGGGatttaaatGGGGATCCGCACAAACGAA SwaI Homologous aox1 TT region corresponding to nt 1217 to 1235
YL4-F CCCCatttaaatGGATCCCCCACACACCATA SwaI Homologous Ptef1 region corresponding to nt 965 to 983
YL4-R GCGCcacgtgCACATGTTGGTCTCCAGCTT PmlI Homologous cyc1 region corresponding to nt 2157 to 2138
YL5-F GGGGgccggcCCAAGTCCAAGGTCTCCAAT NaeI Homologous leu2 region corresponding to nt 86424 to 86443
YL5-R GGGGcacgtgTTTCCGAACCTTACAGTAGAG PmlI Homologous leu2 region corresponding to nt 87940 to 87920
  1. aF forward primer, R reverse primer
  2. bLowercase letters indicates the restriction endonuclease recognized site denoted in the next column
  3. cNucleotide numbering has been taken according to the sequence depicted in the access number mentioned in the section of Materials and methods