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Fig. 1 | AMB Express

Fig. 1

From: Correction to: Autolysis of Pichia pastoris induced by cold

Fig. 1

Structure of pLGC09. Plasmid pLGC09 comprises three modules: a the lytic regulated module is composed of the promoter of chaperonin CCT one complex (Pcctα) that drives expression of eng in P. pastoris and it is finished by aox1 transcription termination (aox1 TT) region; b the selection marker module is composed of the translational elongation factor 1 gene promoter (Ptef1) and of the EM7 synthetic prokaryotic promoter (PEM7) that drive expression of the ble gene in P. pastoris and E. coli respectively, these expressions are finished by cyc1 transcription termination region (cyc1 TT); c the homologous recombination module is composed of the leu2 functional gene including its promoter and transcription termination region; and d the replicon pCR®4Blunt-TOPO® that includes the functional gene bla and pUC origin (oripUC)

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