Fig. 7

Effect of different pH and temperatures on the activity of the recombinant ChOA. a Effect of pH: The residual activity was examined by monitoring H₂O₂ generation at 25 °C. The buffer systems (0.1 M) utilized were glycine–HCl (pH 3.0), citrate-sodium citrate (pH 4.0), CH₃COOH–CH₃COONa (pH 5.0), NaH₂PO₄–Na₂HPO₄ (pH 6.0), Tris–HCl (pH 7.0–9.0), and Na₂CO₃–NaHCO₃ (pH 10.0–11.0). b Effect of temperature: enzyme activity was assayed in 0.1 mM potassium phosphate buffer pH 7.0 at the indicated temperatures