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Table 3 Observed rate constants for the oxidative half reaction. The reactions were performed in 50 mM HEPES–NaOH, pH 7.5 at 25 °C

From: Kinetic characterization of acetone monooxygenase from Gordonia sp. strain TY-5

Without butanone

With butanone

k −OO (mM−1 s−1)a

kox1 (s−1)b

kox2 (s−1)b

kOO-BT (mM−1 s−1)a

k440 (s−1)c

40.1 ± 5.1

0.47 ± 0.01

0.06 ± 0.01 s−1

44.2 ± 9.2

0.80 ± 0.01

  1. aDetermined from the slopes of a plot of the observe rate constants for the absorbance changes at 366 nm versus the concentration of dioxygen (Fig. 8)
  2. bDetermined from fitting a double exponential to the grey single wavelength absorbance trace at 440 nm shown in Fig. 7
  3. cDetermined from fitting a single exponential to the black single wavelength absorbance trace at 440 nm shown in Fig. 7
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