Skip to main content

Table 3 Observed rate constants for the oxidative half reaction. The reactions were performed in 50 mM HEPES–NaOH, pH 7.5 at 25 °C

From: Kinetic characterization of acetone monooxygenase from Gordonia sp. strain TY-5

Without butanone With butanone
k OO (mM−1 s−1)a kox1 (s−1)b kox2 (s−1)b kOO-BT (mM−1 s−1)a k440 (s−1)c
40.1 ± 5.1 0.47 ± 0.01 0.06 ± 0.01 s−1 44.2 ± 9.2 0.80 ± 0.01
  1. aDetermined from the slopes of a plot of the observe rate constants for the absorbance changes at 366 nm versus the concentration of dioxygen (Fig. 8)
  2. bDetermined from fitting a double exponential to the grey single wavelength absorbance trace at 440 nm shown in Fig. 7
  3. cDetermined from fitting a single exponential to the black single wavelength absorbance trace at 440 nm shown in Fig. 7