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Table 1 List of RT-PCR primers used for the amplification of TNF-α, IL-6, COX-2, iNOS, caspase-3, proNGF, p53, bax and bcl-2

From: Anti-inflammatory and anxiolytic activities of Euphorbia hirta extract in neonatal asthmatic rats

S. no Gene name Forward Reverse
1 TNF-α 5′-CCCAGACCCTCACACTCAGAT-3′ 5′-TTGTCCCTTGAAGAGAACCTG-3′
2 IL-6 5′-AAGTTTCTCTCCGCAAGATACTTCCAGCCA-3′ 5′-AGGCAAATTTCCTGGTTATATCCA GTTT-3′
3 COX-2 5′-CCATGTCAAAACCGTGGTGAATG-3′ 5′-ATGGGAGTTGGGCAGTCATCAG-3′
4 iNOS 5′-CTCCATGACTCTCAGCACAGAG-3′ 5′-GCACCGAAGATATCCTCATGAT-3′
5 Caspase-3 5′-TTAATAAAGGTATCCATGGAGAACACT-3′ 5′-TTAGTGATAAAAATAGAGTTCTTTTGTGAG-3′
6 proNGF 5′-CTTCAGCATTCCCTTGACAC-3′ 5′-TGAGCACACACACGCAGGC-3′
7 p53 5′-TAACAGTTCCTGCATGGGCGGC-3′ 5′-AGGACAGGCACAAACACGCACC-3′
8 Bcl-2 5′-CACCCCTGG CATCTTCTCCTT-3′ 5′-AGCGTCTTCAGAGACAGCCAG-3′
9 Bax 5′-TGG AGCTGCAGAGGATGATTG-3′ 5′-GAAGTTGCCGTCAGAAAACATG-3′
10 GAPDH 5′-TCCCTCAAGATTGTCAGCAA-3′ 5′-AGATCCACAACGGATACATT-3′