Table 1 List of RT-PCR primers used for the amplification of TNF-α, IL-6, COX-2, iNOS, caspase-3, proNGF, p53, bax and bcl-2
From: Anti-inflammatory and anxiolytic activities of Euphorbia hirta extract in neonatal asthmatic rats
S. no | Gene name | Forward | Reverse |
|---|---|---|---|
1 | TNF-α | 5′-CCCAGACCCTCACACTCAGAT-3′ | 5′-TTGTCCCTTGAAGAGAACCTG-3′ |
2 | IL-6 | 5′-AAGTTTCTCTCCGCAAGATACTTCCAGCCA-3′ | 5′-AGGCAAATTTCCTGGTTATATCCA GTTT-3′ |
3 | COX-2 | 5′-CCATGTCAAAACCGTGGTGAATG-3′ | 5′-ATGGGAGTTGGGCAGTCATCAG-3′ |
4 | iNOS | 5′-CTCCATGACTCTCAGCACAGAG-3′ | 5′-GCACCGAAGATATCCTCATGAT-3′ |
5 | Caspase-3 | 5′-TTAATAAAGGTATCCATGGAGAACACT-3′ | 5′-TTAGTGATAAAAATAGAGTTCTTTTGTGAG-3′ |
6 | proNGF | 5′-CTTCAGCATTCCCTTGACAC-3′ | 5′-TGAGCACACACACGCAGGC-3′ |
7 | p53 | 5′-TAACAGTTCCTGCATGGGCGGC-3′ | 5′-AGGACAGGCACAAACACGCACC-3′ |
8 | Bcl-2 | 5′-CACCCCTGG CATCTTCTCCTT-3′ | 5′-AGCGTCTTCAGAGACAGCCAG-3′ |
9 | Bax | 5′-TGG AGCTGCAGAGGATGATTG-3′ | 5′-GAAGTTGCCGTCAGAAAACATG-3′ |
10 | GAPDH | 5′-TCCCTCAAGATTGTCAGCAA-3′ | 5′-AGATCCACAACGGATACATT-3′ |