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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: An AA9-LPMO containing a CBM1 domain in Aspergillus nidulans is active on cellulose and cleaves cello-oligosaccharides

Fig. 3

Activity of AN1602 on cellohexaose. a PACE gel showing digestion products from cellohexaose upon AN1602 activity in the presence of 1 mM ascorbic acid. Lane 1—cellohexaose, lane 2—cellohexaose treated with AN1602. The migration standards (M) are cello-oligosaccharides corresponding to Glc2 (cellobiose), Glc3 (cellotriose), Glc4 (cellotetraose) and Glc6 (cellohexaose). b HPAEC chromatogram showing the generation of native and oxidized oligosaccharides after incubating cellohexaose (0.5 mg/mL) with AN1602. A mixture of nonoxidized cello-oligosaccharide (DP2-6) standards in the control run is also shown. Analysis was carried out after treating cellohexaose with AN1602 under similar conditions as in Fig. 2a. nC nanocoulomb

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