Fig. 4

NA and AngII fusion gene expression and recombinant NA-AngII fusion protein production analysis in rBV-NA-AngII transduced HEK293 cells by reverse-transcription PCR (a) and Western blot analysis (b), respectively. cDNA and proteins obtained from a wild type AcMNPV transduced HEK293 cells were (−) controls for a and b, respectively. For reverse transcription PCR, primers specific to neuraminidase and AngII peptide nucleotide sequences were used for PCR of cDNA prepared from the rBA-NA-AngII transduced HEK293 cells. A plasmid with the NA-AngII fusion gene was a (+) control. For Western blot analysis, an anti-NA polyclonal antibody for detection of recombinant neuraminidase protein and an anti-AngII monoclonal antibody for detection of AngII peptides, as a part of the recombinant neuraminidase protein, were used to detect recombinant NA-AngII fusion protein. Arrow indicates recombinant NA-AngII fusion protein