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Fig. 2 | AMB Express

Fig. 2

From: Recombinant neuraminidase pseudotyped baculovirus: a dual vector for delivery of Angiotensin II peptides and DNA vaccine

Fig. 2

NA and AngII fusion gene expression and recombinant NA-AngII fusion protein analysis in the rBV-NA-AngII infected Sf9 insect cells by reverse transcription-PCR (a) and Western blot analysis (b), respectively. For reverse-transcription PCR, primers specific to neuraminidase (NA) and AngII peptides nucleotide sequences (AngII) were used for PCR of cDNA prepared from rBA-NA-AngII infected Sf-9 cells. A plasmid with NA-AngII fusion gene was used as a (+) control for PCR. For Western blot analysis, an anti-NA polyclonal antibody for detection of recombinant neuraminidase protein and an anti-AngII monoclonal antibody for detection of AngII peptides (AngII), as a part of the recombinant neuraminidase protein, were used. Sf9 insect cells infected with wild type AcMNPV was used as a (−) control. Arrow indicates recombinant NA-AngII fusion protein

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