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Table 4 Comparative analysis of different purification strategies for the cellulase from Schizophyllum commune NAIMCC-F-03379

From: Production, purification and characterization of an acid/alkali and thermo tolerant cellulase from Schizophyllum commune NAIMCC-F-03379 and its application in hydrolysis of lignocellulosic wastes

Technique

Purification step

Enzyme activity (IU/mL)

Protein (mg/ml)

Vol. (mL)

Total activity (IU)

Total protein (mg)

Specific activity

Yield (%)

PF

Ammonium sulphate precipitation

Crude

236.4

19.8

500

118,193.9

9882.00

12.0

100

1

60% (NH4)2SO4

1011.7

13.9

13

13,151.8

180.18

73.0

11.1

6.1

Anion exchange chromatography

Crude

236.4

19.8

5

1181.9

98.80

12.0

100

1

400 mM Fraction

242.4

5.0

2

484.9

10.08

48.1

41.0

4.0

Gel filtration chromatography

Crude

236.4

19.8

3

709.2

59.40

12.0

100

1

0 – 10 ml Fraction

103.3

2.3

5

516.6

11.55

44.7

72.8

3.7

ATPS with TX-114

Crude

236.4

19.8

4

945.6

79.20

12.0

100

1

8% (v/v) TX-114

249.6

2.9

3

748.8

8.64

86.7

79.2

7.2

ATPS with MnSO4

Crude

236.4

19.8

2

472.8

39.60

12.0

100

1

22.5% salt 11.3% PEG 8000

93.9

0.8

4

375.8

3.00

125.3

79.5

10.4

  1. Crude enzyme was subjected to each purification methods separately (Single Step Purification). Purification fold with respect to the crude enzyme. The respective enzyme load in each purification technique was mentioned in methodology