Technique | Purification step | Enzyme activity (IU/mL) | Protein (mg/ml) | Vol. (mL) | Total activity (IU) | Total protein (mg) | Specific activity | Yield (%) | PF |
---|---|---|---|---|---|---|---|---|---|
Ammonium sulphate precipitation | Crude | 236.4 | 19.8 | 500 | 118,193.9 | 9882.00 | 12.0 | 100 | 1 |
60% (NH4)2SO4 | 1011.7 | 13.9 | 13 | 13,151.8 | 180.18 | 73.0 | 11.1 | 6.1 | |
Anion exchange chromatography | Crude | 236.4 | 19.8 | 5 | 1181.9 | 98.80 | 12.0 | 100 | 1 |
400Â mM Fraction | 242.4 | 5.0 | 2 | 484.9 | 10.08 | 48.1 | 41.0 | 4.0 | |
Gel filtration chromatography | Crude | 236.4 | 19.8 | 3 | 709.2 | 59.40 | 12.0 | 100 | 1 |
0 – 10 ml Fraction | 103.3 | 2.3 | 5 | 516.6 | 11.55 | 44.7 | 72.8 | 3.7 | |
ATPS with TX-114 | Crude | 236.4 | 19.8 | 4 | 945.6 | 79.20 | 12.0 | 100 | 1 |
8% (v/v) TX-114 | 249.6 | 2.9 | 3 | 748.8 | 8.64 | 86.7 | 79.2 | 7.2 | |
ATPS with MnSO4 | Crude | 236.4 | 19.8 | 2 | 472.8 | 39.60 | 12.0 | 100 | 1 |
22.5% salt 11.3% PEG 8000 | 93.9 | 0.8 | 4 | 375.8 | 3.00 | 125.3 | 79.5 | 10.4 |