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Table 4 Comparative analysis of different purification strategies for the cellulase from Schizophyllum commune NAIMCC-F-03379

From: Production, purification and characterization of an acid/alkali and thermo tolerant cellulase from Schizophyllum commune NAIMCC-F-03379 and its application in hydrolysis of lignocellulosic wastes

Technique Purification step Enzyme activity (IU/mL) Protein (mg/ml) Vol. (mL) Total activity (IU) Total protein (mg) Specific activity Yield (%) PF
Ammonium sulphate precipitation Crude 236.4 19.8 500 118,193.9 9882.00 12.0 100 1
60% (NH4)2SO4 1011.7 13.9 13 13,151.8 180.18 73.0 11.1 6.1
Anion exchange chromatography Crude 236.4 19.8 5 1181.9 98.80 12.0 100 1
400 mM Fraction 242.4 5.0 2 484.9 10.08 48.1 41.0 4.0
Gel filtration chromatography Crude 236.4 19.8 3 709.2 59.40 12.0 100 1
0 – 10 ml Fraction 103.3 2.3 5 516.6 11.55 44.7 72.8 3.7
ATPS with TX-114 Crude 236.4 19.8 4 945.6 79.20 12.0 100 1
8% (v/v) TX-114 249.6 2.9 3 748.8 8.64 86.7 79.2 7.2
ATPS with MnSO4 Crude 236.4 19.8 2 472.8 39.60 12.0 100 1
22.5% salt 11.3% PEG 8000 93.9 0.8 4 375.8 3.00 125.3 79.5 10.4
  1. Crude enzyme was subjected to each purification methods separately (Single Step Purification). Purification fold with respect to the crude enzyme. The respective enzyme load in each purification technique was mentioned in methodology