Production, purification and characterization of an acid/alkali and thermo tolerant cellulase from Schizophyllum commune NAIMCC-F-03379 and its application in hydrolysis of lignocellulosic wastes

AMB Express

Table 2 Experimental design with coded levels of variables used in CCD-RSM with predicted and experimental CMCase and FPase activity for Schizophyllum commune NAIMCC-F-03379

Run	Space type	Coded levels					CMCase		FPase
Run	Space type	X1	X2	X3	X4	X5	Experimental	Predicted	Experimental	Predicted
1	Factorial	− 1	− 1	1	− 1	− 1	60.57	58.49	65.33	78.75
2	Factorial	− 1	− 1	− 1	1	1	40.11	40.98	58.72	51.99
3	Factorial	0	0	1	0	0	85.78	83.89	69.21	64.99
4	Factorial	− 1	− 1	1	1	1	47.17	45.23	64.09	64.42
5	Factorial	0	0	0	1	1	55.81	54.71	61.80	75.96
6	Factorial	0	− 1	− 1	1	− 1	49.90	47.87	169.98	153.70
7	Factorial	0	− 1	1	− 1	0	72.82	74.69	64.18	61.15
8	Factorial	− 1	1	1	1	− 1	46.73	46.65	75.03	69.20
9	Factorial	1	− 1	0	0	− 1	84.99	84.03	70.27	66.48
10	Factorial	− 1	1	0	− 1	0	59.51	61.44	61.27	75.58
11	Factorial	1	0	− 1	− 1	1	88.60	91.59	77.94	80.41
12	Factorial	− 1	− 1	1	1	− 1	47.78	47.67	55.37	51.92
13	Factorial	0	− 1	− 1	− 1	1	72.03	66.39	65.95	64.13
14	Factorial	0	− 1	1	1	1	55.45	57.70	55.81	56.17
15	Factorial	− 1	− 1	1	− 1	1	54.93	57.98	87.63	89.75
16	Factorial	− 1	1	1	− 1	− 1	60.22	61.00	74.67	67.95
17	Factorial	0	− 1	− 1	− 1	− 1	72.21	73.71	63.04	62.30
18	Factorial	0	1	1	1	− 1	45.49	48.45	73.70	89.82
19	Factorial	0	− 1	1	1	− 1	48.75	47.26	52.46	43.50
20	Factorial	1	1	− 1	1	0	65.33	67.38	57.13	59.86
21	Factorial	0	1	− 1	− 1	− 1	68.15	66.18	62.68	68.06
22	Factorial	− 1	− 1	− 1	− 1	− 1	60.04	60.79	58.54	50.31
23	Factorial	− 1	1	1	1	1	47.87	47.86	56.51	55.14
24	Factorial	0	1	1	− 1	1	96.72	93.40	62.42	58.41
25	Factorial	0	1	− 1	0	1	67.97	70.82	59.86	64.76
26	Factorial	− 1	1	− 1	1	− 1	51.05	49.81	54.75	53.63