Effect of four trace elements on Paenibacillus polymyxa Pp-7250 proliferation, activity and colonization in ginseng

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Table 1 Screening of the concentrations of four single trace elements and orthogonal table of trace elements concentration compounds

Trace elements (mg/L)	1	2	3	4	5	6	7	8
Concentration of Cu²⁺, Fe²⁺, Mn²⁺ and Zn²⁺
Cu²⁺	0	10	30	50	100	500	2500	5000
Fe²⁺	0	10	30	50	100	500	2500	5000
Mn²⁺	0	10	30	50	100	500	2500	5000
Zn²⁺	0	50	100	500	1000	2000	3000	4000

Test number	Cu²⁺ (A)	Fe²⁺ (B)	Zn²⁺ (C)	Mn²⁺ (D)
Factors and levels of orthogonal test
1	10	30	50	30
2	10	50	100	50
3	10	100	150	100
4	30	30	100	100
5	30	50	150	30
6	30	100	50	50
7	50	30	150	50
8	50	50	50	100
9	50	100	100	30