Skip to main content

Table 2 Expression and activity analysis between mcSP-MCAP and α-MF-MCAP

From: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Strain

Signal peptide

Crude extract (mg L−1)

Milk clotting (units mL−1)

X-33/Wild-type

–

0

0

X-33/MCAP3

α-MF + mcSP

0

0

X-33/MCAP-5

α-MF

110

249

X-33/MCAP-3

mcSP

200

410

SMD1168/MCAP-3

mcSP

200

415

  1. Date are given as average, n = 3