Lyoprotection and stabilization of laccase extract from Coriolus hirsutus, using selected additives

AMB Express

Table 6 Effect of additives used in the reaction mixture on laccase thermal stability in aqueous medium composed of acetate buffer (0.1 M, pH 5.0), using syringaldazine as substrate

Thermal inactivation parameter	Additive
Thermal inactivation parameter	None	CuSO₄ 0.002% (w/v)	PEG-8000 0.02% (w/v)	Glycerol 0.4% (w/v)
k_t (h⁻¹)^a	0.0178	0.0131	0.0127	0.0138
T₅₀ (h)^b	38.9	52.9	54.6	50.2

^aConstant of inactivation was determined from the first order kinetics behavior of the inactivation effect of increasing incubation time at a specific temperature
^bT₅₀ is defined as the incubation time at the specific temperature in acetate buffer (0.1 M, pH 5.0) required to report a 50% decrease in the initial activity