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Table 4 The effect of selected additives used in the reaction mixture on laccase activity of an enzymatic extract, obtained from Coriolus hirsutus in aqueous medium composed of acetate buffer (0.1 M, pH 5.0), using syringaldazine as substrate

From: Lyoprotection and stabilization of laccase extract from Coriolus hirsutus, using selected additives

Additive inactivation parameter

Additive

CuSO4

PEG-8000

Glycerol

k ainactivation

0.441

0.088

0.103

C b1/2

1.6

7.8

6.7

  1. aThe rate constant of inactivation (kinactivation), expressed as 1/% (w/v) of the additive per reaction volume, was estimated by plotting the ln percentage residual laccase activity versus the percentage of additive in the reaction trial for laccase
  2. bThe C1/2, expressed in % (w/v) of the additive per reaction volume, defined as the additive concentration at which 50% of the initial activity was reached