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Table 3 The effect of selected additives used in the reaction mixture on the laccase specific activity of an enzymatic extract from Coriolus hirsutus

From: Lyoprotection and stabilization of laccase extract from Coriolus hirsutus, using selected additives

Additive

Relative concentration (%)a

Relative specific activity (%)b

None

–

100

CuSO4

0.002

87.7 (7.0)c

PEG-8000

0.02

117.2 (6.9)

Glycerol

0.4

118.2 (9.4)

  1. aRelative concentration of the additive was expressed in percent (w/v), was the one at which the laccase specific activity was at its optimum
  2. bRelative specific activity was calculated and defined as the concentration of oxidized syringaldazine (nmol product/mg protein/min) in the reaction trial containing the optimum additive concentration relative to that without additive
  3. cRelative percentage standard deviation was defined as the standard deviation of laccase triplicate trial divided by their respective means, multiplied by 100
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