Lyoprotection and stabilization of laccase extract from Coriolus hirsutus, using selected additives

AMB Express

Table 2 Long-term stability of laccase activity of the lyophilized enzymatic extract from Coriolus hirsutus, prepared with 2.5% (w/v) mannitol or dextran 15–25 kDa

Storage (week)	− 80 °C			4 °C			25 °C
Storage (week)	Control^b	Mannitol	Dextran	Control^b	Mannitol	Dextran	Control^b	Mannitol	Dextran
Relative residual laccase activity (%)^a
0	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0	100.0
1	85.0	96.9	57.1	48.1	95.3	19.8	46.3	49.7	0.0
2	78.6	95.8	50.6	28.2	28.3	21.0	24.4	25.6	0.0
3	78.5	101.9	47.0	8.8	33.9	9.0	16.4	26.0	0.0
4	56.6	96.2	38.5	8.5	38.9	4.4	17.8	24.7	0.0

^aRelative residual laccase activity (%) was calculated by dividing the specific activity (nmol product/mg protein/min) of a given sample at defined storage time and temperature to that at time 0, multiplied by 100. The enzyme activity was assayed with syringaldazine as substrate
^bControl was defined as the lyophilized enzyme without additive