Table 3 Kinetic parameters of LP-tan, SS-tan, LP-tan P356G, SS-tan G384P and the variants
Methyl gallate | Tannic acid | |||||
|---|---|---|---|---|---|---|
Km (mM) | kcat (s−1) | kcat/Km (s−1mM−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1mM−1) | |
LP-tan | 0.67 ± 0.15 | 99.4 ± 11.4 | 158.4 ± 35.1 | 1.23 ± 0.4 | 62.6 ± 13.5 | 51.9 ± 16.0 |
LP-tan P356G | 1.3 ± 0.29 | 79.8 ± 8.9 | 66.4 ± 9.4 | 0.94 ± 0.35 | 66.9 ± 8.0 | 72.2 ± 7.5 |
LP-tan variant | 1.1 ± 0.35 | 115.5 ± 16.6 | 95.3 ± 11.9 | 0.53 ± 0.18 | 69.2 ± 3.5 | 135.6 ± 4.0 |
SS-tan | 3.3 ± 0.38 | 69.9 ± 7.5 | 21.2 ± 1.9 | 0.21 ± 0.09 | 64.7 ± 3.6 | 311.1 ± 23.6 |
SS-tan G384P | 2.4 ± 0.31 | 70.3 ± 11.3 | 29.3 ± 4.5 | 0.41 ± 0.11 | 85.1 ± 3.3 | 197.6 ± 15.8 |
SS-tan variant | 3.5 ± 0.51 | 60.7 ± 4.6 | 18.3 ± 1.1 | 0.58 ± 0.28 | 104.6 ± 6.7 | 189.3 ± 8.7 |