Fig. 1

Amino acid sequence alignment of tannase from L. plantarum and S. sviceus. The secondary structure elements of LP-tan are indicated. The conserved residues of the catalytic triad are indicated by red rectangle. The flap part of LP-tan is indicated by black rectangle. The residues of the catalytic triad are indicated by red triangles and those involved in substrates binging are indicated by black triangles. The flap-like domain of SS-tan is indicated by red rectangle. The black dot indicated the mutation of sandwich structure