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Table 2 Substrate spectrum of putative BS115 ω-TA

From: Enantiomer discrimination in β-phenylalanine degradation by a newly isolated Paraburkholderia strain BS115 and type strain PsJN

Amino-donor

Relative activity (%)

ee

rac-3-amino-3-phenylpropionic acid (β-PA)

100

> 99%

rac-3-amino-4-phenylbutyric acid (β-homophenylalanine)

0

rac-3-amino-3-(4-methoxyphenyl)propionic acid

46

> 96%

(S)-3-amino-3-(4-hydroxyphenyl)propionic acid (β-tyrosine)

69

rac-3-amino-3-(4-fluorophenyl)propionic acid

61

> 99%

rac-3-amino-3-(4-nitrophenyl)propionic acid

34

> 91%

rac-3-amino-3-(4-chlorophenyl)propionic acid

19

> 99%

  1. The reactions were carried out in 50 mM TRIS-buffer at pH 8, with an acceptor molecule concentration of 5 mM. The total protein-concentration of the cell lysate was set to 0.2 mg/mL. ee% was determined after 30 min of reaction time