Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method

AMB Express

Table 6 Analysis regression and variance results

Source	Sum of squares	df	Mean squares	F value	p-value prob > F	Significance
Model	32,355.03	9	3595.00	249.81	< 0.0001	**
X ₁	621.28	1	621.28	43.17	0.0003	**
X ₂	3465.28	1	3465.28	240.79	< 0.0001	**
X ₃	60.50	1	60.50	4.20	0.0795
X ₁ ·X ₂	484.00	1	484.00	33.63	0.0007	**
X ₁ ·X ₃	663.06	1	663.06	46.07	0.0003	**
X ₂ ·X ₃	517.56	1	517.56	35.96	0.0005	**
X ²₁	13,275.04	1	13,275.04	922.45	< 0.0001	**
X ²₂	4020.25	1	4020.25	279.36	< 0.0001	**
X ²₃	6661.27	1	6661.27	462.87	< 0.0001	**
Residual	100.74	7	14.39
Lack of fit	43.94	3	14.65	1.03	0.4683
Pure error	56.80	4	14.20
Cor total	32,455.76	16
R² = 0.9969, \({\text{R}}_{\text{Adj}}^{2}\) = 0.9929, \({\text{R}}_{\text{Pred}}^{2}\) = 0.9756