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Table 5 Box–Behnken design

From: Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method

Run

X1 [shaking speed (rmp)]

X2 [culture temperature (°C)]

X3 (pH)

Total viable B. mucilaginous (108 Obj/mL)

1

150

28

7.0

0.44 ± 0.01

2

200

28

6.5

1.15 ± 0.02

3

200

28

6.5

1.16 ± 0.03

4

200

32

6.0

0.77 ± 0.02

5

250

32

6.5

0.27 ± 0.01

6

250

28

6.0

0.21 ± 0.01

7

200

24

6.0

0.09 ± 0.00

8

150

32

6.5

0.68 ± 0.01

9

200

32

7.0

0.58 ± 0.01

10

200

28

6.5

1.18 ± 0.02

11

150

28

6.0

0.11 ± 0.01

12

250

28

7.0

0.03 ± 0.00

13

250

24

6.5

0.11 ± 0.00

14

200

24

7.0

0.35 ± 0.01

15

200

28

6.5

1.20 ± 0.02

16

200

28

6.5

1.10 ± 0.02

17

150

24

6.5

0.09 ± 0.00