Table 1 Factors and levels assessed in single-factor tests
Level | Factor | |||||
|---|---|---|---|---|---|---|
Concentration (%) | pH | Loaded liquid (mL/250 mL) | Inoculum (v:v, %) | Culture temperature (°C) | Shaking speed (rpm) | |
1 | 0.0625 | 5.0 | 15 | 0.5 | 20 | 50 |
2 | 0.125 | 5.5 | 30 | 1 | 24 | 100 |
3 | 0.25 | 6.0 | 60 | 2 | 28 | 150 |
4 | 0.5 | 6.5 | 90 | 4 | 32 | 200 |
5 | 1 | 7.0 | 120 | 8 | 36 | 250 |
6 | 2 | 7.5 | 150 | 16 | – | – |
7 | 8.0 | – | – | – | – | |