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Table 3 ANOVA for Box–Behnken design

From: Optimization of rPDT fusion protein expression by Escherichia coli in pilot scale fermentation: a statistical experimental design approach

Source

Sum of squares

df

Mean square

F value

p-value prob > F

Model

5.562E+007

9

6.180E+006

73.81

< 0.0001

A-T

4.735E+007

1

4.735E+007

565.51

< 0.0001

B-DO

48615.39

1

48615.39

0.58

0.4710

C-IPTG

2.827E+005

1

2.827E+005

3.38

0.1088

AB

4.387E+005

1

4.387E+005

5.24

0.0559

AC

45926.63

1

45926.63

0.55

0.4830

BC

1900.39

1

1900.39

0.023

0.8845

A2

4.177E+006

1

4.177E+006

49.88

0.0002

B2

8.466E+005

1

8.466E+005

10.11

0.0155

C2

1.741E+006

1

1.741E+006

20.80

0.0026

Residual

5.862E+005

7

83736.38

  

 Lack of fit

3.009E+005

3

1.003E+005

1.41

0.3637

 Pure error

2.853E+005

4

71316.17

  

Cor total

5.621E+007

16

Square