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Table 1 Polymerization of catechol under different experimental conditions (laccase amount; OD at 400 nm; total content of free OH, calculated by Folin–Ciocalteu method and by 1H NMR; average degree of polymerization and dispersity)

From: Exploring PEGylated and immobilized laccases for catechol polymerization

  Activity (U/mL) OD—8 h (400 nm) Amount of OH groups (Mn; Mw)a Average DPa DM (dispersity)
By Folin–Ciocalteu method By 1H NMR
Free/native LAC 100 0.926 0.515 0.500 748; 776 7 1.03
Free/PEGylated LAC 1.452 0.293 0.310 833; 849 8 1.02
Immobilized/native LAC 0.857 0.192 1088; 1118 10 1.03
Immobilized/PEGylated LAC 1.301 0.202 0.110 1460; 1498 14 1.02
Epoxy-PEG-LAC 1.003 0.075 0.320 1570; 1600 15 1.02
  1. aAverage degree of polymerization calculated by MALDI-TOF analysis; 1 U is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of substrate (ABTS) per minute per mg of protein used