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Table 1 Polymerization of catechol under different experimental conditions (laccase amount; OD at 400 nm; total content of free OH, calculated by Folin–Ciocalteu method and by 1H NMR; average degree of polymerization and dispersity)

From: Exploring PEGylated and immobilized laccases for catechol polymerization

 

Activity (U/mL)

OD—8 h (400 nm)

Amount of OH groups

(Mn; Mw)a

Average DPa

DM (dispersity)

By Folin–Ciocalteu method

By 1H NMR

Free/native LAC

100

0.926

0.515

0.500

748; 776

7

1.03

Free/PEGylated LAC

1.452

0.293

0.310

833; 849

8

1.02

Immobilized/native LAC

0.857

0.192

–

1088; 1118

10

1.03

Immobilized/PEGylated LAC

1.301

0.202

0.110

1460; 1498

14

1.02

Epoxy-PEG-LAC

1.003

0.075

0.320

1570; 1600

15

1.02

  1. aAverage degree of polymerization calculated by MALDI-TOF analysis; 1 U is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of substrate (ABTS) per minute per mg of protein used