Fig. 7

Bi-modal cultivation of S. cerevisiae growing in SD medium with glucose as sole carbon source. Cell dry weight concentrations, pH, glucose; ethanol and FCdiene concentrations of fed-batch fermentation (0–24 h) with combined glucose pulse in the KLF2000 fermenter (working volume 1.8 L) are shown. In the fed-batch phase, the pH was regulated using ammonia solution (6.65 M), in the batch phase the pH was unregulated