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Table 4 Analysis of variance (ANOVA) of all response variables

From: Statistical modelling for optimized lyophilization of Lactobacillus acidophilus strains for improved viability and stability using response surface methodology

Responses SS DF MS F P (Pr > F)
Survival rate (%)
 Model 524.74 2 266.37 89.35 < 0.0001**
 A-Sucrose 138.87 1 138.87 47.29 < 0.0001
 B-Reconstituted skim milk 385.88 1 385.88 131.42 < 0.0001
 Residual 29.36 10 2.94   
 Lack of fit 29.18 6 4.86 106.58 0.0002*
 Pure error 0.18 4 0.05   
 Cor total 554.11 12    
VCAL
 Model 37.77 3 12.59 92.85 < 0.0001**
 A-Sucrose 10.81 1 10.81 79.70 < 0.0001
 B-Reconstituted skim milk 11.87 1 11.87 87.58 < 0.0001
 AB 15.09 1 15.09 111.27 < 0.0001
 Residual 1.22 9 0.14   
 Lack of fit 1.22 5 0.24   
 Pure error 0.00 4 0.00   
 Cor total 38.99 12    
VCAL 1 month
 Model 40.80 3 13.60 70.71 < 0.0001**
 A-Sucrose 11.09 1 11.09 57.68 < 0.0001
 B-Reconstituted skim milk 10.74 1 10.74 55.85 < 0.0001
 AB 18.96 1 18.96 98.60 < 0.0001
 Residual 1.73 9 0.19   
 Lack of fit 1.73 5 0.35   
 Pure error 0.00 4 0.00   
 Cor total 42.53 12    
VCAL 6 months
 Model 50.86 3 16.95 48.39 < 0.0001**
 A-Sucrose 13.57 1 13.57 38.73 0.0002
 B-Reconstituted skim milk 13.72 1 13.72 39.17 0.0001
 AB 23.57 1 23.57 67.28 < 0.0001
 Residual 3.15 9 0.35   
 Lack of fit 3.15 5 0.63   
 Pure error 0.00 4 0.00   
 Cor total 54.02 12    
VCAL 12 months
 Model 61.37 3 20.46 39.64 < 0.0001**
 A-Sucrose 16.20 1 16.20 31.40 0.0003
 B-Reconstituted skim milk 16.45 1 16.45 31.88 0.0003
 AB 28.72 1 28.72 55.66 < 0.0001
 Residual 4.64 9 0.52   
 Lack of fit 4.64 5 0.93   
 Pure error 0.00 4 0.00   
 Cor total 66.02 12    
VCAL 18 months
 Model 61.03 3 20.34 41.44 < 0.0001**
 A-Sucrose 15.69 1 15.69 31.97 0.0003
 B-Reconstituted skim milk 15.94 1 15.94 32.47 0.0003
 AB 29.39 1 29.39 59.87 < 0.0001
 Residual 4.42 9 0.49   
 Lack of fit 4.42 5 0.88   
 Pure error 0.00 4 0.00   
 Cor total 65.45 12    
VCAL 24 months
 Model 60.57 3 20.19 41.68 < 0.0001**
 A-Sucrose 15.48 1 15.48 31.95 0.0003
 B-Reconstituted skim milk 15.73 1 15.73 32.48 0.0003
 AB 29.36 1 29.36 60.62 < 0.0001
 Residual 4.36 9 0.48   
 Lack of fit 4.36 5 0.87   
 Pure error 0.00 4 0.00   
 Cor total 64.93 12    
  1. DF degrees of freedom, SS sum of squares, MS mean square, F and P (Pr > F) F and P values, respectively
  2. ** P < 0.0001, very significant; * P < 0.005, significant