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Table 4 Analysis of variance (ANOVA) of all response variables

From: Statistical modelling for optimized lyophilization of Lactobacillus acidophilus strains for improved viability and stability using response surface methodology

Responses

SS

DF

MS

F

P (Pr > F)

Survival rate (%)

 Model

524.74

2

266.37

89.35

< 0.0001**

 A-Sucrose

138.87

1

138.87

47.29

< 0.0001

 B-Reconstituted skim milk

385.88

1

385.88

131.42

< 0.0001

 Residual

29.36

10

2.94

  

 Lack of fit

29.18

6

4.86

106.58

0.0002*

 Pure error

0.18

4

0.05

  

 Cor total

554.11

12

   

VCAL

 Model

37.77

3

12.59

92.85

< 0.0001**

 A-Sucrose

10.81

1

10.81

79.70

< 0.0001

 B-Reconstituted skim milk

11.87

1

11.87

87.58

< 0.0001

 AB

15.09

1

15.09

111.27

< 0.0001

 Residual

1.22

9

0.14

  

 Lack of fit

1.22

5

0.24

  

 Pure error

0.00

4

0.00

  

 Cor total

38.99

12

   

VCAL 1 month

 Model

40.80

3

13.60

70.71

< 0.0001**

 A-Sucrose

11.09

1

11.09

57.68

< 0.0001

 B-Reconstituted skim milk

10.74

1

10.74

55.85

< 0.0001

 AB

18.96

1

18.96

98.60

< 0.0001

 Residual

1.73

9

0.19

  

 Lack of fit

1.73

5

0.35

  

 Pure error

0.00

4

0.00

  

 Cor total

42.53

12

   

VCAL 6 months

 Model

50.86

3

16.95

48.39

< 0.0001**

 A-Sucrose

13.57

1

13.57

38.73

0.0002

 B-Reconstituted skim milk

13.72

1

13.72

39.17

0.0001

 AB

23.57

1

23.57

67.28

< 0.0001

 Residual

3.15

9

0.35

  

 Lack of fit

3.15

5

0.63

  

 Pure error

0.00

4

0.00

  

 Cor total

54.02

12

   

VCAL 12 months

 Model

61.37

3

20.46

39.64

< 0.0001**

 A-Sucrose

16.20

1

16.20

31.40

0.0003

 B-Reconstituted skim milk

16.45

1

16.45

31.88

0.0003

 AB

28.72

1

28.72

55.66

< 0.0001

 Residual

4.64

9

0.52

  

 Lack of fit

4.64

5

0.93

  

 Pure error

0.00

4

0.00

  

 Cor total

66.02

12

   

VCAL 18 months

 Model

61.03

3

20.34

41.44

< 0.0001**

 A-Sucrose

15.69

1

15.69

31.97

0.0003

 B-Reconstituted skim milk

15.94

1

15.94

32.47

0.0003

 AB

29.39

1

29.39

59.87

< 0.0001

 Residual

4.42

9

0.49

  

 Lack of fit

4.42

5

0.88

  

 Pure error

0.00

4

0.00

  

 Cor total

65.45

12

   

VCAL 24 months

 Model

60.57

3

20.19

41.68

< 0.0001**

 A-Sucrose

15.48

1

15.48

31.95

0.0003

 B-Reconstituted skim milk

15.73

1

15.73

32.48

0.0003

 AB

29.36

1

29.36

60.62

< 0.0001

 Residual

4.36

9

0.48

  

 Lack of fit

4.36

5

0.87

  

 Pure error

0.00

4

0.00

  

 Cor total

64.93

12

   
  1. DF degrees of freedom, SS sum of squares, MS mean square, F and P (Pr > F) F and P values, respectively
  2. ** P < 0.0001, very significant; * P < 0.005, significant