Skip to main content

Advertisement

Table 1 Comparison of the transformation results for Pionier cauliflower cultivar

From: High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

Rhizobium species R. radiobacter R. rhizogenes
Strain EHA 105 LBA 4404 A4 ATCC 15834
Transformation efficiency 11
% of hypocotyls with at least 1 shoot
9
% of hypocotyls with at least 1 shoot
72
% of seedlings with at least 1 hairy root
9
% of seedlings with at least 1 hairy root
% of transgenic explants confirmed in GUS assay 8a
% of transgenic hypocotyls confirmed in GUS assay
2a
% of transgenic hypocotyls confirmed in GUS assay
55
% of transgenic hairy roots confirmed in GUS assay
15
% of transgenic hairy roots confirmed in GUS assay
Regeneration efficiency 0.28
Average number of shoots per hypocotyl
0.09
Average number of shoots per hypocotyl
2.1
Average number of buds per callus
0.5
Average number of buds per callus
Rooting efficiency of transgenic shoots 0
Average number of rooted shoots per their total number
0
Average number of rooted shoots per their total number
90
Average number of rooted shoots per their total number
78
Average number of rooted shoots per their total number
  1. aTrace staining (spots) at the end of hypocotyls