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Table 1 Comparison of the transformation results for Pionier cauliflower cultivar
From: High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes
| Rhizobium species | R. radiobacter | R. rhizogenes | ||
|---|---|---|---|---|
| Strain | EHA 105 | LBA 4404 | A4 | ATCC 15834 |
| Transformation efficiency |
11 % of hypocotyls with at least 1 shoot |
9 % of hypocotyls with at least 1 shoot |
72 % of seedlings with at least 1 hairy root |
9 % of seedlings with at least 1 hairy root |
| % of transgenic explants confirmed in GUS assay |
8a % of transgenic hypocotyls confirmed in GUS assay |
2a % of transgenic hypocotyls confirmed in GUS assay |
55 % of transgenic hairy roots confirmed in GUS assay |
15 % of transgenic hairy roots confirmed in GUS assay |
| Regeneration efficiency |
0.28 Average number of shoots per hypocotyl |
0.09 Average number of shoots per hypocotyl |
2.1 Average number of buds per callus |
0.5 Average number of buds per callus |
| Rooting efficiency of transgenic shoots |
0 Average number of rooted shoots per their total number |
0 Average number of rooted shoots per their total number |
90 Average number of rooted shoots per their total number |
78 Average number of rooted shoots per their total number |
