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Fig. 2 | AMB Express

Fig. 2

From: High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

Fig. 2

Molecular analysis of transformants. a Electrophoresis of genomic DNA: lane 1—a wild type plant (WT), lanes 2–4—independent transgenic lines (TL1, TL2, TL3); b detection of the coding sequence of the gusPlus gene (488 bp) in B. oleracea var. botrytis transgenic and wild-type plants. Lane 1—a positive control (PC, plasmid 1305.2), lane 2—a molecular weight marker (GeneRuler 1 kb DNA Ladder), lanes 3–7—independent transgenic lines (TL1, TL2, TL3, TL4, TL5), lane 8—a wild-type plant (WT); c SDS-PAGE separation of proteins from the transgenic cauliflower plants. Lane 15—independent transgenic lines (TL1, TL2, TL3, TL4, TL5), lane 6—molecular weight marker (M—GPB 260 kDa Protein Marker), lane 7—a wild type plant (WT); d detection of the β-glucuronidase protein in the total extractable protein isolates derived both from B. oleracea var. botrytis wild type and transgenic plants. Lane 1—molecular weight marker (M—GPB 260 kDa Protein Marker), lane 2—a wild type plant protein isolate (WT), lanes 3,4—independent transgenic lines (L1, L2), lane 5—positive control (L3). Contrary to the wild type, in the case of transgenic plant extracts a positive signal was observed (about 80 kDa)

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