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Fig. 1 | AMB Express

Fig. 1

From: High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

Fig. 1

The process of R. rhizogenes-mediated transformation, regeneration and analysis of the B. oleracea var. botrytis (Pionier cultivar) plants (scheme for the A4 strain). a Cauliflower seed germination; b hypocotyls of the derooted seedlings after bacteria dropping; c hairy root induction on the hypocotyls; d an individual hairy root; eg stages of the transgenic hairy root multiplication; h, i the GUS assay on the hairy root clones, wild type negative control and the tested probe respectively; j indirect organogenesis on the transgenic callus obtained on the hairy root cultures; k shoot development; l rooting of the transgenic cauliflower shoots; mo histochemical analysis of the cauliflower leaves, wild type negative control and the tested probes respectively

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