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A. Primers used in the PCR amplified disruption cassettes for gene disruption
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D-ERG3_Fw
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ATTTCTATCTTTCTTATCAATTCGTTTTTTCATTCACTTGTCAGCTGAAGCTTCGTACGC
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D-ERG3_Rv
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TCTTGAACGTGAAAGAAAGAAAAAAGATGAGACAAACAAGATAGGCCACTAGTGGATCTG
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D-NCE103_Fw
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TACAAATTTCAATTATTACACATCAGACAGCTGAAGCTTCGTACGC
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D-NCE103_Rv
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CCCCGTCTACTTTGTAAATGTCTTTCTATTTCAATGAATGGTAGGCCACTAGTGGATCTG
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D-TRP1_Fw
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GTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGACAGCTGAAGCTTCGTACGC
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D-TRP1_Rv
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CTATTTCTTAGCATTTTTGACGAAATTTGCTATTTTGTGCATAGGCCACTAGTGGATCTG
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B. Primers used for the confirmation of successful gene disruption in yeast chromosome
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C-ERG3_Fw1
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CGAAACGACGCCTTTTGTTGCGATTGTCG
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C-ERG3_Rv1
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ATTTGTGTGCTTCTCTTGACGTTCGTTCG
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C-ERG3_Fw2
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TTCAACAAGTTTCAATAGCTCATCAGTCG
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C-ERG3_Rv2
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GAAATCTTGGGCATTTTAAAGCTTCCAGC
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C-NCE103_Fw1
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GTCACCATGACGCTTATCAAGCC
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C-NCE103_Rv1
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ATCGGGCGTTTACCGTATCGC
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C-NCE103_Fw2
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CTACACCTGGGGTCATGATTAGCC
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C-NCE103_Rv2
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GACATTTGCTGGATCACAGACCG
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C. Primers used in the PCR amplification of the GAL1 promoter derivative fused with hCAII
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pGAL1.4 Fw
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TACAGCTAAGACTACAAAACGGATTAGAAGCCGCCG
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pGAL1.3 Fw
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TACAGCTAAGACTACAAACCGAGCGGGCGACAGCCC
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pGAL1.2 Fw
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TACAGCTAAGACTACAAACCGACGGAAGACTCTCCTC
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pGAL1.1 Fw
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TACAGCTAAGACTACAAAGCAGATGTGCCTCGCGCC
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NotI_hCAII Rv
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TTTTCCTTTTGCGGCCGCTTTTTTCCTTTTATTTATCATCATCATCTTTG
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