Table 3 Oligonucleotide primers used in this study

A. Primers used in the PCR amplified disruption cassettes for gene disruption

 D-ERG3_Fw

ATTTCTATCTTTCTTATCAATTCGTTTTTTCATTCACTTGTCAGCTGAAGCTTCGTACGC

 D-ERG3_Rv

TCTTGAACGTGAAAGAAAGAAAAAAGATGAGACAAACAAGATAGGCCACTAGTGGATCTG

 D-NCE103_Fw

TACAAATTTCAATTATTACACATCAGACAGCTGAAGCTTCGTACGC

 D-NCE103_Rv

CCCCGTCTACTTTGTAAATGTCTTTCTATTTCAATGAATGGTAGGCCACTAGTGGATCTG

 D-TRP1_Fw

GTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGACAGCTGAAGCTTCGTACGC

 D-TRP1_Rv

CTATTTCTTAGCATTTTTGACGAAATTTGCTATTTTGTGCATAGGCCACTAGTGGATCTG

B. Primers used for the confirmation of successful gene disruption in yeast chromosome

 C-ERG3_Fw1

CGAAACGACGCCTTTTGTTGCGATTGTCG

 C-ERG3_Rv1

ATTTGTGTGCTTCTCTTGACGTTCGTTCG

 C-ERG3_Fw2

TTCAACAAGTTTCAATAGCTCATCAGTCG

 C-ERG3_Rv2

GAAATCTTGGGCATTTTAAAGCTTCCAGC

 C-NCE103_Fw1

GTCACCATGACGCTTATCAAGCC

 C-NCE103_Rv1

ATCGGGCGTTTACCGTATCGC

 C-NCE103_Fw2

CTACACCTGGGGTCATGATTAGCC

 C-NCE103_Rv2

GACATTTGCTGGATCACAGACCG

C. Primers used in the PCR amplification of the GAL1 promoter derivative fused with hCAII

 pGAL1.4 Fw

TACAGCTAAGACTACAAAACGGATTAGAAGCCGCCG

 pGAL1.3 Fw

TACAGCTAAGACTACAAACCGAGCGGGCGACAGCCC

 pGAL1.2 Fw

TACAGCTAAGACTACAAACCGACGGAAGACTCTCCTC

 pGAL1.1 Fw

TACAGCTAAGACTACAAAGCAGATGTGCCTCGCGCC

 NotI_hCAII Rv

TTTTCCTTTTGCGGCCGCTTTTTTCCTTTTATTTATCATCATCATCTTTG