Fig. 5

Determination of the minimal effective dose of the tested compounds. Serial dilutions of either AZA (0–25 µM), avicennin (0–500 µM) or FK506 (0–10 µM) were added in wells of a 96-well plate containing 0.5 × 10⁵ cells mL⁻¹ of AS03(pGAL1.1_hCAII) cells and incubated at 30 °C under a high- or low-CO₂ condition. a The growth of the yeast strain treated with AZA was determined by the OD₆₆₀ value prior to adding resazurin into each well. b Then, after 4 h, the OD₅₇₂ and OD₆₀₀ values were measured and expressed as the OD₅₇₂/OD₆₀₀ ratio. c The colorimetric results of the assay for three tested compounds were also observed visually. Data are shown as (a, b) the mean ± 1 SD derived from three independent repeats, where *, ** and *** represent a significant difference at p < 0.05, p < 0.01 and p < 0.001 levels, respectively, compared to that of the untreated control group, and c a representative image of three independent repeats