Skip to main content
Fig. 4 | AMB Express

Fig. 4

From: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors

Fig. 4

Optimization of the initial cell concentration using the REMA. A suspension of the yeast strain AS03(pGAL1.1_hCAII) at varying final concentrations of 1 × 106, 5 × 105, 1 × 105, 5 × 104 and 1 × 104 cells mL−1 in 100 µL of SG + Ade + Leu + His medium were added to each well of a 96-well plate with varying concentrations of AZA (0, 6.25, 12.5 and 25 µM). The plates were incubated at 30 °C for 24 h whereupon resazurin was added and 4 h later the OD572 and OD600 values were measured and expressed as a the OD572/OD600 ratio and b visual observation. Data are shown as a the mean ± 1 SD derived from three independent repeats, where *, ** and *** represent a significant difference at p < 0.05, p < 0.01 and p < 0.001 levels, respectively, compared to that of the untreated control group, and b representative image of three independent repeats

Back to article page