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Fig. 3 | AMB Express

Fig. 3

From: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors

Fig. 3

Drug (AZA) sensitivity of the AS03 strain expressing varying levels of hCAII. Tenfold dilutions of cell suspension of the yeast strain AS03 carrying either the empty pRS414 vector or one of pGAL1.1_hCAII, pGAL1.2_hCAII, pGAL1.3_hCAII or pGAL1.4_hCAII at concentrations of 106, 105, 104 and 103 cells mL−1 were spotted onto the surface of SG + Ade + Leu + His agar plates containing 0, 3.13, 6.25 or 12.5 μM of AZA and incubated at 30 °C for 3–4 days under a low CO2 condition. Images shown are representative from three independent repeats

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