Fig. 1

Expression level of hCAII under the control of variously modified GAL1 promoters. The drug-sensitive AS03 yeast strain harboring either the empty pRS414 vector or one of the GAL1 promoter derivatives containing either one, two, three or four copies of the Gal4-binding sites (pGAL1.1_hCAII, pGAL1.2_hCAII, pGAL1.3_hCAII or pGAL1.4_hCAII, respectively) was cultivated under a GAL1-induced condition for 24 h before harvesting. a Protein extracts were prepared and subjected to Western blot analysis using anti-Flag to detect hCAII and anti-β-actin to detect β-actin. Blots shown are representative of those seen from three independent repeats. b Total RNA was prepared and converted to cDNA prior to the qRT-PCR analysis of the hCAII mRNA expression level. Data are expressed as the relative level of hCAII mRNA to actin mRNA and shown as the mean expression level, ± 1 SD, and derived from three independent repeats. **Significant difference at p < 0.01 compared to that of the transformant with the pGAL1.4_hCAII plasmid