Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

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Table 5 Decolorization (%) of different kinds of synthetic dyes by Il-DyP4 and Il-MnP6

Nr.	Dye	λ_max (nm)	Concentration (μM)	Il-DyP4^a			Il-MnP6^a
Nr.	Dye	λ_max (nm)	Concentration (μM)	pH 3.5	pH 4	pH 4.5	pH 3.5	pH 4	pH 4.5
Anthraquinone dyes
1	Reactive blue 4	598	200	79.28	81.92	82.01	1.87	7.24	0.96
2	Reactive blue 5	600	100	74.06	82.73	83.61	12.40	18.07	8.26
3	Reactive blue 19	595	100	78.39	74.97	70.01	2.78	5.71	5.90
Azo dyes
4	Direct sky blue 5B	598	100	80.62	81.45	77.91	16.95	59.72	63.75
5	Reactive black 5	598	50	60.57	59.30	40.86	0.22	0	0.69
6	Acid red 18	506	100	43.07	43.73	38.04	6.09	7.14	3.53
7	Reactive violet 5	570	100	92.16	90.81	80.96	19.97	21.32	1.10
8	Methyl orange	464	50	33.12	30.21	29.60	11.21	9.32	1.95
9	Direct yellow 8	392	25	2.62	4.66	2.04	0	0	0
10	Orange G	478	50	18.38	21.28	25.39	33.80	34.72	22.90
11	Orange yellow II	484	50	28.65	27.94	36.59	7.58	4.68	2.18
12	Orange yellow IV	445	50	62.55	60.07	60.16	13.23	10.40	4.26
13	Congo red	498	10	0	38.69	52.77	0	0	0
Phenazine dyes
14	Neutral red	550	100	22.50	24.15	31.09	0	2.40	5.68
Triphenylmethane dyes
15	Malachite green	618	50	84.80	82.43	72.53	24.74	28.03	24.69
Aniline dyes
16	Basic fuchsin	542	25	45.10	39.75	23.86	3.46	3.63	0.25

^aPercentage of dye decolorization after 30 min is based on the observed decrease in absorbance at λ_max