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Table 2 Kinetic parameters—Km (μM), kcat (s−1), and kcat/Km (s−1M−1) of Il-DyP4 and Il-MnP6 for substrates oxidation obtained based on the optimal pH and temperature (°C)

From: Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

  Il-DyP4 Il-MnP6
pH Temperature Kinetic constants pH Temperature Kinetic constants
H2O a2 3.5 35 K m 163 ± 20.8 4.5 30 K m 194 ± 18
   k cat (1.2 ± 0.08) × 104    k cat 879 ± 48
   kcat/Km (7.6 ± 0.5) × 107    kcat/Km (4.5 ± 0.3) × 106
ABTS 3.5 35 K m 62 ± 11 3.5 45 K m 83 ± 6.5
   k cat 8356 ± 747    k cat 245 ± 7
   kcat/Km (1.3 ± 0.1) × 108    kcat/Km (3.0 ± 0.09) × 106
Mn2+ 4.5 35 K m 2687 ± 463 4.5 30 K m 129 ± 8
   k cat 806 ± 94    k cat 369 ± 6.1
   kcat/Km (3.0 ± 0.4) × 105    kcat/Km (2.9 ± 0.3) × 106
DMP 4 35 K m 58 ± 3 4.5 55 K m 53 ± 8.6
   k cat 4896 ± 131    k cat 989 ± 100
   kcat/Km (8.4 ± 0.2) × 107    kcat/Km (1.9 ± 0.2) × 107
Guaiacol 4 45 K m 24 ± 7.5 4 45 K m 2.6 ± 0.6
   k cat 413 ± 34    k cat 184 ± 7
   kcat/Km (1.7 ± 0.1) × 107    kcat/Km (7.1 ± 0.3) × 107
VA 3.5 45 K m (2.1 ± 0.98) × 104 b K m c
   k cat 108 ± 47    k cat
   kcat/Km (5.2 ± 2.3) × 103    kcat/Km
RBlue 19 4.5 35 K m 133 ± 34 3 35 K m 81 ± 39.5
   k cat 5345 ± 921    k cat 7.3 ± 2.6
   kcat/Km (4.0 ± 0.7) × 107    kcat/Km (9.0 ± 3.2) × 104
RBlack 5 4 35 K m 159 ± 61 3 35 K m 4.4 ± 0.7
   k cat 267 ± 96    k cat 0.06 ± 0.003
   kcat/Km (1.7 ± 0.6) × 106    kcat/Km (1.5 ± 0.07) × 104
  1. Means and 95% confidence limits
  2. aActivities were measured with 1.25 mM ABTS and 1 mM Mn2+ by Il-DyP4 and Il-MnP6, respectively
  3. bNot detected
  4. cExperimental condition: [VA] = 1 mM, [MnP] = 25 nM, [H2O2] = 0.1 mM, [MnSO4] = 1.0 mM, [Tartrate buffer] = 100 mM, pH = 3.5, temperature = 45 °C, reaction time = 60 min