AMB Express

Table 2 Kinetic parameters—K_m (μM), k_cat (s⁻¹), and k_cat/K_m (s⁻¹M⁻¹) of Il-DyP4 and Il-MnP6 for substrates oxidation obtained based on the optimal pH and temperature (°C)

From: Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

	Il-DyP4				Il-MnP6
	pH	Temperature	Kinetic constants		pH	Temperature	Kinetic constants
H₂O ^a₂	3.5	35	K _m	163 ± 20.8	4.5	30	K _m	194 ± 18
			k _cat	(1.2 ± 0.08) × 10⁴			k _cat	879 ± 48
			k_cat/K_m	(7.6 ± 0.5) × 10⁷			k_cat/K_m	(4.5 ± 0.3) × 10⁶
ABTS	3.5	35	K _m	62 ± 11	3.5	45	K _m	83 ± 6.5
			k _cat	8356 ± 747			k _cat	245 ± 7
			k_cat/K_m	(1.3 ± 0.1) × 10⁸			k_cat/K_m	(3.0 ± 0.09) × 10⁶
Mn²⁺	4.5	35	K _m	2687 ± 463	4.5	30	K _m	129 ± 8
			k _cat	806 ± 94			k _cat	369 ± 6.1
			k_cat/K_m	(3.0 ± 0.4) × 10⁵			k_cat/K_m	(2.9 ± 0.3) × 10⁶
DMP	4	35	K _m	58 ± 3	4.5	55	K _m	53 ± 8.6
			k _cat	4896 ± 131			k _cat	989 ± 100
			k_cat/K_m	(8.4 ± 0.2) × 10⁷			k_cat/K_m	(1.9 ± 0.2) × 10⁷
Guaiacol	4	45	K _m	24 ± 7.5	4	45	K _m	2.6 ± 0.6
			k _cat	413 ± 34			k _cat	184 ± 7
			k_cat/K_m	(1.7 ± 0.1) × 10⁷			k_cat/K_m	(7.1 ± 0.3) × 10⁷
VA	3.5	45	K _m	(2.1 ± 0.98) × 10⁴	–^b	–	K _m	–^c
			k _cat	108 ± 47			k _cat	–
			k_cat/K_m	(5.2 ± 2.3) × 10³			k_cat/K_m	–
RBlue 19	4.5	35	K _m	133 ± 34	3	35	K _m	81 ± 39.5
			k _cat	5345 ± 921			k _cat	7.3 ± 2.6
			k_cat/K_m	(4.0 ± 0.7) × 10⁷			k_cat/K_m	(9.0 ± 3.2) × 10⁴
RBlack 5	4	35	K _m	159 ± 61	3	35	K _m	4.4 ± 0.7
			k _cat	267 ± 96			k _cat	0.06 ± 0.003
			k_cat/K_m	(1.7 ± 0.6) × 10⁶			k_cat/K_m	(1.5 ± 0.07) × 10⁴

Means and 95% confidence limits
^aActivities were measured with 1.25 mM ABTS and 1 mM Mn²⁺ by Il-DyP4 and Il-MnP6, respectively
^bNot detected
^cExperimental condition: [VA] = 1 mM, [MnP] = 25 nM, [H₂O₂] = 0.1 mM, [MnSO₄] = 1.0 mM, [Tartrate buffer] = 100 mM, pH = 3.5, temperature = 45 °C, reaction time = 60 min

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