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Fig. 7 | AMB Express

Fig. 7

From: Identification of genome-wide binding sites of heat shock factor 1, Hsf1, under basal conditions in the human pathogenic yeast, Candida albicans

Fig. 7

Analysis of cellular localization of Hsf1—Microscopy images of Hsf1 cellular localization in a wildtype [BPS (−)] cells, b iron deprived [BPS (+)] cells and c heat shock treated cells. The fluorescent images are shown alongside quantified intensity plot. The topmost panel in each section represents staining with primary antibody (anti-Hsf1 coupled with secondary antibody labeled with FITC visualized as green fluorescence). The middle panel represents nuclear staining with DAPI. The bottom panel is the merged Fig. The fluorescence intensity of the selected transversal section of the cell (arrow line) was quantified and represented as intensity plot, where the length and direction are expressed as distance on the x-axis. Two peaks in the intensity plot in a and b corresponding to cytoplasmic localization of Hsf1 was observed. The dip in fluorescence depicting Hsf1 concentration in the nucleus in a and b panel is due to the lesser amount of Hsf1 in the nucleus as compared to the cytoplasm. The dip is more prominent in case of BPS (+) cells. The sharp peak in case of heat shock c corresponds to increased nuclear localization of Hsf1 upon heat shock conditions

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