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Table 1 The conditions used in organosolv (OS) and steam explosion (SE) pretreatment together with the composition of the biomass obtained.

From: A comparative study of the enzymatic hydrolysis of batch organosolv-pretreated birch and spruce biomass

  Biomass extracted with 50% (v/v) ethanol Biomass extracted with 60% (v/v) ethanol
Time 60 min, 182 °C 103 min, 182 °C 60 min, 182 °C
Particle size 1 mm 4 mm 1 mm 4 mm 1 mm 4 mm
H2SO4 (w/v) 0% 1% 0% 1% 0% 1% 0% 1% 0% 1% 0% 1%
Birch
 Cellulose 62.2 59.6 61.4 57.6 63.7 57.8 61.4 62.4 59.0 60.7 62.1 63.8
 Hemicellulose 3.0 0.5 4.6 0.3 2.6 0.4 2.7 0.2 3.3 1.1 4.3 1.1
 Lignin 16.0 24.1 14.6 21.8 17.5 25.9 15.8 23.7 16.8 15.7 13.8 16.0
 Total (wt%) 81.2 84.1 80.6 79.8 83.8 84.2 79.9 86.3 79.0 77.5 80.2 80.9
Spruce
 Cellulose 52.5 56.1 53.8 58.0 54.5 55.9 56.1 55.2 50.3 69.1 49.1 67.3
 Hemicellulose 5.0 2.0 6.4 2.4 5.6 3.5 7.3 2.8 13.2 1.2 14.5 2.8
 Lignin 29.9 31.7 26.3 29.8 28.4 37.8 25.8 40.9 23.7 25.0 26.0 24.2
 Total (wt%) 87.4 89.8 86.5 90.1 88.5 97.2 89.2 98.9 87.2 95.3 89.6 94.3
  1. Composition of untreated birch (by wt%): 34.7% cellulose, 31.2% hemicellulose, and 18.7% lignin; composition of untreated spruce: 37.6% cellulose, 27.4% hemicellulose, and 32.6% lignin; composition of steam exploded birch: 57.2% cellulose, 12.1% hemicellulose, and 27.1% lignin (Matsakas et al. 2018); composition of steam exploded spruce: 38.2% cellulose, 53.1% lignin