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Table 1 The conditions used in organosolv (OS) and steam explosion (SE) pretreatment together with the composition of the biomass obtained.

From: A comparative study of the enzymatic hydrolysis of batch organosolv-pretreated birch and spruce biomass

 

Biomass extracted with 50% (v/v) ethanol

Biomass extracted with 60% (v/v) ethanol

Time

60 min, 182 °C

103 min, 182 °C

60 min, 182 °C

Particle size

1 mm

4 mm

1 mm

4 mm

1 mm

4 mm

H2SO4 (w/v)

0%

1%

0%

1%

0%

1%

0%

1%

0%

1%

0%

1%

Birch

 Cellulose

62.2

59.6

61.4

57.6

63.7

57.8

61.4

62.4

59.0

60.7

62.1

63.8

 Hemicellulose

3.0

0.5

4.6

0.3

2.6

0.4

2.7

0.2

3.3

1.1

4.3

1.1

 Lignin

16.0

24.1

14.6

21.8

17.5

25.9

15.8

23.7

16.8

15.7

13.8

16.0

 Total (wt%)

81.2

84.1

80.6

79.8

83.8

84.2

79.9

86.3

79.0

77.5

80.2

80.9

Spruce

 Cellulose

52.5

56.1

53.8

58.0

54.5

55.9

56.1

55.2

50.3

69.1

49.1

67.3

 Hemicellulose

5.0

2.0

6.4

2.4

5.6

3.5

7.3

2.8

13.2

1.2

14.5

2.8

 Lignin

29.9

31.7

26.3

29.8

28.4

37.8

25.8

40.9

23.7

25.0

26.0

24.2

 Total (wt%)

87.4

89.8

86.5

90.1

88.5

97.2

89.2

98.9

87.2

95.3

89.6

94.3

  1. Composition of untreated birch (by wt%): 34.7% cellulose, 31.2% hemicellulose, and 18.7% lignin; composition of untreated spruce: 37.6% cellulose, 27.4% hemicellulose, and 32.6% lignin; composition of steam exploded birch: 57.2% cellulose, 12.1% hemicellulose, and 27.1% lignin (Matsakas et al. 2018); composition of steam exploded spruce: 38.2% cellulose, 53.1% lignin