Run | Parameter | Factor levels | ||
---|---|---|---|---|
Low | Medium | High | ||
1 | Biomass (OD600 value) | 2 | 4 | 6 |
2 | l-Methionine concentration (w/v) | 0.1% | 0.5% | 1.0% |
3 | Agitation rate (rpm) | 0 | 80 | 120 |
4 | Temperature (°C) | 25 | 30 | 38 |
5 | pH (100 mM phosphate buffer) | 4 | 5 | 6 |
6 | Nitrogen supplementation (w/v) | 0% | 0.1% | 0.3% |
7 | Yeast extract (w/v) | 0% | 0.1% | 0.3% |
8 | Mn2+ supplementation (mM) | 0 | 1 | 10 |