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Table 1 Factor levels investigated for each parameter

From: Effects of physicochemical parameters on volatile sulphur compound formation from l-methionine catabolism by non-growing cells of Kluyveromyces lactis

Run Parameter Factor levels
Low Medium High
1 Biomass (OD600 value) 2 4 6
2 l-Methionine concentration (w/v) 0.1% 0.5% 1.0%
3 Agitation rate (rpm) 0 80 120
4 Temperature (°C) 25 30 38
5 pH (100 mM phosphate buffer) 4 5 6
6 Nitrogen supplementation (w/v) 0% 0.1% 0.3%
7 Yeast extract (w/v) 0% 0.1% 0.3%
8 Mn2+ supplementation (mM) 0 1 10
  1. #6: nitrogen = diammonium phosphate