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Table 1 Factor levels investigated for each parameter

From: Effects of physicochemical parameters on volatile sulphur compound formation from l-methionine catabolism by non-growing cells of Kluyveromyces lactis

Run

Parameter

Factor levels

Low

Medium

High

1

Biomass (OD600 value)

2

4

6

2

l-Methionine concentration (w/v)

0.1%

0.5%

1.0%

3

Agitation rate (rpm)

0

80

120

4

Temperature (°C)

25

30

38

5

pH (100 mM phosphate buffer)

4

5

6

6

Nitrogen supplementation (w/v)

0%

0.1%

0.3%

7

Yeast extract (w/v)

0%

0.1%

0.3%

8

Mn2+ supplementation (mM)

0

1

10

  1. #6: nitrogen = diammonium phosphate