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AMB Express
Fig. 6 | AMB Express

Fig. 6

From: Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

Fig. 6

Lane 1, immunoblotting of the supernatant after treatment of the whole surface displayed recombinant bacterial cell with sortase. Lane 2, immunoblotting of the pellet of the recombinant bacteria after treatment with sortase, almost one band corresponding to the complete chimeric protein is observable. This shows that a few amount of the chimeric protein remains on the surface of the bacteria after treatment with sortase

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